Journal: Antioxidants

Article Title: Unraveling the Protective Role of Oleocanthal and Its Oxidation Product, Oleocanthalic Acid, against Neuroinflammation

doi: 10.3390/antiox13091074

Figure Lengend Snippet: TLR4 and CD14 surface expression of BV-2 cells. BV-2 cells were pre-treated for 2 h with 10 µM of OL and OA and then exposed to 100 ng/mL of LPS for 24 h. Surface expression of TLR4 and CD14 was determined by flow cytometry, as reported in Materials and Methods. Each bar represents the mean ± SEM of three independent experiments (one unit per group). Panel A shows the surface expression of TLR4; Panel B shows the surface expression of CD14. Data were analyzed by one-way ANOVA followed by Tukey’s test. * p < 0.05 with respect to CTRL and ° p < 0.05 with respect to LPS.

Article Snippet: The resulting cell pellet was then washed with PBS, centrifuged at 300× g for 5 min, and resuspended with PE-conjugated mouse anti-CD14 antibody (Miltenyi Biotec, cat. no. 130-115-558, 51429 Bergisch Gladbach, Germany), 1:50 dilution in PBS + 1% BSA, then incubated for 45 min in the dark at 4 °C.

Techniques: Expressing, Flow Cytometry

Journal: Antioxidants

Article Title: Unraveling the Protective Role of Oleocanthal and Its Oxidation Product, Oleocanthalic Acid, against Neuroinflammation

doi: 10.3390/antiox13091074

Figure Lengend Snippet: Molecular docking results of OL and OA (green and purple respectively) in m CD14 (PDB ID 1WWL). Residues detected through mutagenesis studies to be responsible for LPS binding and LPS signaling are shown in tan and gray spheres, respectively. Residues involved in the binding of OL and OA are highlighted.

Article Snippet: The resulting cell pellet was then washed with PBS, centrifuged at 300× g for 5 min, and resuspended with PE-conjugated mouse anti-CD14 antibody (Miltenyi Biotec, cat. no. 130-115-558, 51429 Bergisch Gladbach, Germany), 1:50 dilution in PBS + 1% BSA, then incubated for 45 min in the dark at 4 °C.

Techniques: Mutagenesis, Binding Assay

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet: Antibody master mix

Article Snippet: Anti-Mouse CD14 (Sa14-2)-156Gd—100 Tests , Fluidigm , SKU# 3156009B.

Techniques:

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD14 (Sa14-2)-156Gd—100 Tests , Fluidigm , SKU# 3156009B.

Techniques: Purification, Recombinant, Red Blood Cell Lysis, Centrifugation, Staining, Electron Microscopy, Antibody Labeling, Software, Sterility, Spectrophotometry